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Tissues were homogenized in RLT buffer (QIAGEN), a lysis buffer that inactivates RNases and facilitates RNA extraction, using a bead homogenizer for 3 min at maximum speed (Qiagen, TissueLyser II)

It remains a widely referenced compound in experimental peptide research and analytical laboratory environments

The mTOR directly phosphorylates 4E-BP1 and p70S6K which then phosphorylate S6 to initiate protein translation step

Akcan U, Karabulut S, Ismail Kkali C, akir S, Tzn E

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